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b per tm reagent  (Thermo Fisher)


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    Structured Review

    Thermo Fisher b per tm reagent
    B Per Tm Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+per/Phosphate/pm42000960-75-5-8
    Average 99 stars, based on 1 article reviews
    b per tm reagent - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Protein Extraction:

    Article Title: Rational truncation of the p40 aggregation-inducing domain enables tailored formation of functional bacterial inclusion bodies for biocatalysis
    Article Snippet: .. The obtained insoluble pellet containing target IBs was washed once with 1x PBS and then treated with B-PER® (in phosphate buffer) protein extraction reagent (Thermo Fisher Scientific). .. Briefly, 1 g of insoluble pellet was treated with 4 mL B-PER® supplemented with 5 U of Benzonase (Sigma-Aldrich) and incubated at room temperature for 15 min with gentle agitation.

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea
    Article Snippet: Briefly, a single colony of the recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD 600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/iai.00406-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 14 M ar ch 2 02 5 by 2 40 0: 1a 00 :b 03 0: f6 3: b8 08 :3 93 3: f1 12 :c 70 8. recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Centrifugation:

    Article Title: De novo designed inhibitor confers protection against lethal toxic shock
    Article Snippet: .. Cells were pelleted via centrifugation at 4000xg for 5 min and then lysed by incubation in B-PER (Thermo Fisher) with 0.1 mg/ml lysozyme, 10 μg/ml DNAse I and 1 mM PMSF for 15 min. Lysates were then cleared by centrifugation at 4000xg for 15 min and then applied to 50 μL Ni-NTA resin (Thermo Fisher) in a 96-well 25 μm plate. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea
    Article Snippet: Briefly, a single colony of the recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD 600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/iai.00406-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 14 M ar ch 2 02 5 by 2 40 0: 1a 00 :b 03 0: f6 3: b8 08 :3 93 3: f1 12 :c 70 8. recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Incubation:

    Article Title: De novo designed inhibitor confers protection against lethal toxic shock
    Article Snippet: .. Cells were pelleted via centrifugation at 4000xg for 5 min and then lysed by incubation in B-PER (Thermo Fisher) with 0.1 mg/ml lysozyme, 10 μg/ml DNAse I and 1 mM PMSF for 15 min. Lysates were then cleared by centrifugation at 4000xg for 15 min and then applied to 50 μL Ni-NTA resin (Thermo Fisher) in a 96-well 25 μm plate. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea
    Article Snippet: Briefly, a single colony of the recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD 600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/iai.00406-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 14 M ar ch 2 02 5 by 2 40 0: 1a 00 :b 03 0: f6 3: b8 08 :3 93 3: f1 12 :c 70 8. recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Bacteria:

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea
    Article Snippet: Briefly, a single colony of the recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD 600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/iai.00406-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 14 M ar ch 2 02 5 by 2 40 0: 1a 00 :b 03 0: f6 3: b8 08 :3 93 3: f1 12 :c 70 8. recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Sonication:

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea
    Article Snippet: Briefly, a single colony of the recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD 600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Article Title: Heterologous prime-boost immunization of two-component vaccine candidate PWDVax protected pigs against F18 enterotoxigenic Escherichia coli post-weaning diarrhea.
    Article Snippet: Month XXXX Volume 0 Issue 0 10.1128/iai.00406-2411 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 14 M ar ch 2 02 5 by 2 40 0: 1a 00 :b 03 0: f6 3: b8 08 :3 93 3: f1 12 :c 70 8. recombinant E. coli strain was cultured in 200 mL 2× YT medium supplemented with 30 μg/mL Kanamycin (Sigma, St. Louis, MO, USA) at 37°C until OD600 reached 0.5–0.7. .. After four more hours of incubation, bacteria were harvested by centrifugation and lysed with B-PER (bacteria protein extraction reagent, in PBS, Thermo Fisher Scientific, Waltham, MA, USA), lysozyme (10 mg/mL), and sonication. ..

    Western Blot:

    Article Title: A comprehensive framework for the interpretation of TTN missense variants
    Article Snippet: .. For Western blot solubility analysis, the cell pellet was resuspended in 20mM HEPES pH7.5, 300mM NaCl and 15mM imidazole, supplemented with cOmplete protease inhibitor (Roche) and then lysed with B-Per (Thermo Fisher) supplemented with lysozyme (0.1mg/mL) and Benzonase (40U/mL, Merck) for 30 minutes at room temperature. ..

    Solubility:

    Article Title: A comprehensive framework for the interpretation of TTN missense variants
    Article Snippet: .. For Western blot solubility analysis, the cell pellet was resuspended in 20mM HEPES pH7.5, 300mM NaCl and 15mM imidazole, supplemented with cOmplete protease inhibitor (Roche) and then lysed with B-Per (Thermo Fisher) supplemented with lysozyme (0.1mg/mL) and Benzonase (40U/mL, Merck) for 30 minutes at room temperature. ..

    Protease Inhibitor:

    Article Title: A comprehensive framework for the interpretation of TTN missense variants
    Article Snippet: .. For Western blot solubility analysis, the cell pellet was resuspended in 20mM HEPES pH7.5, 300mM NaCl and 15mM imidazole, supplemented with cOmplete protease inhibitor (Roche) and then lysed with B-Per (Thermo Fisher) supplemented with lysozyme (0.1mg/mL) and Benzonase (40U/mL, Merck) for 30 minutes at room temperature. ..

    Article Title: Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells
    Article Snippet: .. After harvest, pellets were lysed with lysis buffer containing B-PER (Thermo Scientific, 89821), 1.0 mg/mL lysozyme (MilliporeSigma, L6876), Halt protease inhibitor cocktail (Thermo Scientific, 78430), and Benzonase nuclease (Millipore, E1014) on a rocking shaker for 15 min at room temperature. ..

    Lysis:

    Article Title: Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells
    Article Snippet: .. After harvest, pellets were lysed with lysis buffer containing B-PER (Thermo Scientific, 89821), 1.0 mg/mL lysozyme (MilliporeSigma, L6876), Halt protease inhibitor cocktail (Thermo Scientific, 78430), and Benzonase nuclease (Millipore, E1014) on a rocking shaker for 15 min at room temperature. ..



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